中华五角海星体腔液蛋白质分离技术初探

运用聚丙烯酰胺凝胶电泳方法分析中华五角海星体腔液蛋白组分,探究低丰度蛋白样品的初步分离鉴定方法。研究结果表明,中华五角海星体腔液部分蛋白含量处于凝胶电泳的最小检测浓度范围。通过简易样品浓缩处理、上样前样品处理及电泳缓冲液选择等条件优化,实现了对此类量少,低丰度样品良好的分离效果。     

Comparison of defence ability against the white spot syndrome virus between Fenneropenaeus chinensis and Marsupenaeus japonicus

The defence ability of Fenneropenaeus chinensis and Marsupenaeus japonicus against the intrusion of the white spot syndrome virus (WSSV) was compared after injecting WSSV intramuscularly by recording cumulative mortality, diagnosing the virus and examining variations in immunological parameters including the total haemocyte counts (THCs), phagocytic percentage (PP), plasma protein concentration (PPC), phenoloxidase (PO) and nitric oxide synthase (NOS) activity. The results showed that the variations in the immunological parameters of F. chinensis and M. japonicus showed similar trends. The THCs of the two species decreased significantly postchallenge of WSSV. The virus was detected at 78 h in M. japonicus and at 42 h in F. chinensis after infection, which was in correlation with the accumulative mortality, and the variations in PO, PP, NOS and PPC in the two species. All shrimps of F. chinensis in the mortality experiment died within 66 h, much more quickly than M. japonicus, whose cumulative mortality reached 100% after 198 h. In conclusion, the immune system of M. japonicus has a stronger resistant ability to antagonize and endure the invasion of WSSV than that of F. chinensis.     

Preparation and Characterization of Protein Hydrolysates From Little Loligo Squid (Uroteuthis chinensis)

Research on the little loligo squid (Uroteuthis chinensis) protein hydrolyzed by papain and Flavourzyme and characterization of hydrolysates was carried out in this article. The optimum hydrolysis conditions of papain were obtained by orthogonal experiments, and the influence factors on the degree of hydrolysis such as enzyme/substrate ratio, hydrolysis time, pH, and temperature were investigated. The effect of Flavourzyme on the degree of hydrolysis was also studied. The characteristics of freeze-dried hydrolysates such as yield, moisture, nitrogen recovery, molecular mass distribution, and amino acid composition were determined. The results showed that papain and Flavourzyme could hydrolyze the little loligo squid protein efficiently, and the degree of hydrolysis was up to 25.82% under the optimum hydrolysis conditions. The average molecular mass of hydrolysates obtained with papain and Flavourzyme was 1,246 Da, and the low molecular mass peptides were the main fraction of hydrolysates. The yield, moisture, and nitrogen recovery of freeze-dried hydrolysates was 19.8, 4.4, and 74.2%, respectively. Compared with suggested patterns of amino acid requirements by the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), the hydrolysates of the little loligo squid had high nutritional value, and was a potential nutritious supplement used in various food products.     

五味子实生苗地下横走茎形态发生研究

对五味子实生苗地下横走茎的形态发生、发育过程进行了研究。结果表明:1 a生五味子子叶腋芽可发育为地下横走茎,但其发育较缓慢,在8月下旬至9月初才发育成地下横走茎,第1片真叶腋芽和第2片真叶腋芽当年均可以发育成地下横走茎;2 a生和多年生五味子植株基部的越冬芽都会发育成地下横走茎;无论是子叶腋芽还是越冬芽在转变初期与一般芽形态相似,但经过伸长生长后,其茎尖开始弯曲以适应地下生长。地下横走茎的发生部位较集中,常呈轮生状。较高节位芽的超显微结构与子叶叶芽存在较大差异,说明五味子的芽能否形成地下横走茎在其形成的早期可能就已经决定。     

斑节对虾白斑综合症病毒部分基因组文库及核酸探针检测法

通过分离纯化白斑综合症病毒（ＷＳＳＶ）粒子，抽提病毒ＤＮＡ。用限制性内切酶ＥｃｏＲⅠ或ＳａｌⅠ酶切后，克隆入质粒ｐＢｌｕｅｓｃｒｉｐｔⅡＫＳ中，从而建立了ＷＳＳＶ部分基因组文库。估计ＷＳＳＶ基因组ＤＮＡ在１６５ｋｂ以上。将ＷＳＳＶ ＥｃｏＲⅠ克隆片段标记制备为探针。进行Ｓｏｕｔｈｅｒｎ杂交、打点杂交和原位杂交，其结果证明了克隆片段对ＷＳＳＶ特异，并为检测ＷＳＳＶ提供了方法。通过对部分基因组文库序列     

葱斑潜蝇的生物学特性研究

描述了葱斑潜蝇成虫、卵、幼虫、蛹的生物学特性     

用PAPD方法分析太湖大银鱼、太湖新银鱼和寡齿新银鱼的亲缘关系

１９９６￣１９９７年２年中在太湖６个水域采集到大银鱼（Ｐｒｏｔｏｓａｌａｎｘ ｃｈｉｎｅｎｓｉｓ）、太湖新银鱼（Ｎｅｏｓａｌａｎｘ ｒｅｇａｎｉｕｓ）和寡齿新银鱼（Ｎｅｏｓａｌａｎｘ ｏｌｉｇｏｄｏｎｔｉｓ）并发现其中有２个样品的ＲＡＰＤ图谱与其它样品的图谱有很大差异，进一步ＤＮＡ分析结果表明，这２个样品不属于大银鱼，太湖新银鱼和寡齿新银鱼的其它种银鱼，表明太湖中可能存在５种银鱼。     

不同密度的羊草种群结构及其光照分布的研究

本文报道了耕翻后自然更新的羊草种群不同密度条件下垂直结构及其光照分布特征。结果表明，地上生物量和叶面积指数均以种群中部最大，向上或向下皆逐渐减小。在三种密度中，生物量，叶面积指数，株高，茎高和叶片含水量均随着密度的增大而升高。比叶重和单株绿叶数度增加而降低。     

猕猴桃16个优良品种（变种）果实主要经济性状的研究

猕猴桃１６个优良品种（变种）果实主要经济性状研究表明，地处金衡盆地东南边缘的武义县，适宜种植和开发布鲁诺、ＫＨ－５两个优良品种，具经济性状好、果大、风味浓、丰产、供应期长等特点。